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島根大学農学部研究報告 Volume 15
published_at 1981-12-15
葉緑体の脂質分解酵素処理ならびに脱脂質葉緑体の特性
Lipolytic Enzyme Treatment of chloroplasts and Characterization of Lipid-Depleted Chloroplasts
Hirayama Osamu
Nomotobori Takashi
Morita Kohkichi
Okuda Toshihiko
Ichihara Minoru
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Potato galactolipase treatment of spinach chloroplasts were investigated. The enzyme reaction was inhibited with Mg<Cl>___2, bovine serum albumin and sucrose, to varying levels with their specific action on the chloroplast grana stacking. The reaction increased in three steps with increase of temperature, suggesting that there are different localization and existing forms of lipids. By the enzyme treatment and subsequent centrifugation on density-gradient column, lipid-depleted chloroplasts were prepared. Analysis revealed that the preparation retained nearly half portion of the intial electron flows, but H^+ uptake and phosphorylation activities were completely lost.
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