葉緑体の脂質分解酵素処理ならびに脱脂質葉緑体の特性

島根大学農学部研究報告 Volume 15 Page 56-60 published_at 1981-12-15
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Title
葉緑体の脂質分解酵素処理ならびに脱脂質葉緑体の特性
Title
Lipolytic Enzyme Treatment of chloroplasts and Characterization of Lipid-Depleted Chloroplasts
Title Transcription
ヨウリョクタイ ノ シシツ ブンカイ コウソ ショリ ナラビニ ダツ シシツ ヨウリョクタイ ノ トクセイ
Creator
Hirayama Osamu
Nomotobori Takashi
Morita Kohkichi
Okuda Toshihiko
Ichihara Minoru
Source Title
島根大学農学部研究報告
Bulletin of the Faculty of Agriculture, Shimane University
Volume 15
Start Page 56
End Page 60
Journal Identifire
ISSN 0370940X
Descriptions
Potato galactolipase treatment of spinach chloroplasts were investigated. The enzyme reaction was inhibited with Mg<Cl>___2, bovine serum albumin and sucrose, to varying levels with their specific action on the chloroplast grana stacking. The reaction increased in three steps with increase of temperature, suggesting that there are different localization and existing forms of lipids. By the enzyme treatment and subsequent centrifugation on density-gradient column, lipid-depleted chloroplasts were prepared. Analysis revealed that the preparation retained nearly half portion of the intial electron flows, but H^+ uptake and phosphorylation activities were completely lost.
Language
eng
Resource Type departmental bulletin paper
Publisher
島根大学農学部
Shimane University, Faculty of Agriculture
Date of Issued 1981-12-15
Access Rights open access
Relation
[NCID] AN00108015