The writters studied on the antimicrobial components contained in the crude drugs. Cochliobolus miyabeanus (S. Ito et Kuribayashi) Drechsler KU-13 and Bacillus subtilis (Ehrenberg) Cohn IFO-3033 were used as the test microorganisms. Among the ninetytwo samples used in the study, fifty-one samples (55.4%) showed antibacterial activity and twenty-two samples (23.9%) showed antifungal activity. Asenyaku (Uncaria gambir Roxb.), Aroe (Aloe ferox Mill. etc.), Botanpi (Paeonia moutan Sims.), Daio (Rheum palmatum L. etc.), Keihi (Cinnamomum cassia Blume etc.), Kikka (Chrysanthemum morifolium Ramat.), Kujin (Sophora angustifolia Sieb. et Zucc.), Nikkei (Cinnamomum sieboldi Ohwi), and Shikon (Lithospermum officinale L. var. erythrorhizon Maxim.) showed both of the activities to bacteria and fungi.
From the eleven samples such as Asenyaku, Botanpi, Boui (Sinomenium acutum Rehder et Wilson etc.), Daio, Obaku (Phellodendron amurense Rupr.), Ryokyo (Alpinia officinarum Hance), Shikon, Shukushanin (Amomum xanthioides Wall.), Tanba-ouren (Coptis japonica Makino var. dissecta Nakai), and Uwaurushi (Arctostaphylos uva-ursi Sprengel), antifungal substances were extracted using organic solvents.