Bulletin of the Faculty of Agriculture, Shimane University

Faculty of Agriculture, Shimane University

ISSN:0370-940X

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Bulletin of the Faculty of Agriculture, Shimane University 18

1984-12-20 発行

固定化チアミナーゼIを利用したチアミンおよびそのリン酸エステル類の自動定量法

Automatic Determination of Thiamine and Its Phosphates by the Use of Immobilized Thiaminase I

Mochida, Kazuo

Nakamura, Toshiie

Fujita, Keiji

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An automatic method for the determination of thiamine andits phosphates by the application of immobilized thiaminase I was examined.
Thiaminase I catalyzed the base-exchange reaction of the thiamine phosphates as well as thiamine with pyridine, forming heteropyrithiamine(HPT). The contents of thiamine and its phsphates were determined by automatically analyzing HPT formed by the enzyme reaction. It was considered that this system was suitable for the determination of total thiamine.

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