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島根農科大学研究報告 Volume 10
published_at 1962-03-31
好気性桿菌の核酸産生
On the Production of Nucleic Acids by Aerobic Spore-forming Bacteria
Iwahara Shojiro
Matsumoto Muneto
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The paper deals with the production of the nucleic acids in the cultural filtrates of the natto bacteria and other strains of Bacillus group, and with the purification method of the nucleoprotein in the cultural filtrates.
When the organisms were grown on the synthetic medium, remarkable amounts of ultraviolet absorbing substances were produced in the cultural filfrates and increased with the growth of the organisms.
These ultraviolet absorbing substances contain about 20 per cent of acid insoluble nucleoprotein containing 16.67 per cent of DNA, 6.94 per cent of RNA and about 50 per cent of amino acids, and contain uracil as the main base of nucleic acid.
The changes of the amounts of DNA in the cultural filtrates of the organisms were also investigated.
When the organisms were grown on the synthetic medium, remarkable amounts of ultraviolet absorbing substances were produced in the cultural filfrates and increased with the growth of the organisms.
These ultraviolet absorbing substances contain about 20 per cent of acid insoluble nucleoprotein containing 16.67 per cent of DNA, 6.94 per cent of RNA and about 50 per cent of amino acids, and contain uracil as the main base of nucleic acid.
The changes of the amounts of DNA in the cultural filtrates of the organisms were also investigated.
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