Bulletin of the Faculty of Agriculture, Shimane University

Faculty of Agriculture, Shimane University

ISSN:0370-940X

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Use this link to cite this item : https://ir.lib.shimane-u.ac.jp/1833

Bulletin of the Faculty of Agriculture, Shimane University 19

1985-12-20 発行

ダイコン幼植物体における4-チオウリジン代謝生成物のペルオキシダーゼによる酸化

Peroxidase-Catalyzed Oxidation of 4-Thiouridine-Metabolites in Radish Seedlings

Shibata, Hitoshi

Ochiai, Hideo

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Peroxidase (1.11.1.7) purified from Japanese-radish (Raphanus sativus) seedlings catalyzed the oxidation of 4-thiouridine, a sulfur-containing nucleoside, to 4-thiouridine disulfide in the presence of H_2O_2. 4-Thiouridine-metabolites such as 4-thiouracil, 4-thio-UMP, 4-thio-UDP, 4-thio-UTP and 4-thio-UDP glucose also were the substrate of the peroxidase reaction. Acidic peroxidase isoenzymes were mainly responsible for the 4-thiouridine oxidation. An increased 4-thiouridine-oxidase activity in the seedlings germinated and grown with the nucleoside was observed. These result suggest that the oxidation of 4-thiouridine and of its metabolites by peroxidase is not beyond the bounds of possibility in 4-thiouridine-cultured radish seedlings.

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NCID

AN00108015

d0030019n012.pdf 807 KB

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