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Title Transcription
ダイコン ヨウショク ブッタイ ニオケル 4チオウリジン タイシャ セイセイブツ ノ ペルオキシダーゼ ニヨル サンカ
Title Alternative (English)
Peroxidase-Catalyzed Oxidation of 4-Thiouridine-Metabolites in Radish Seedlings
File
language
eng
Author
Shibata, Hitoshi
Ochiai, Hideo
Description
Peroxidase (1.11.1.7) purified from Japanese-radish (Raphanus sativus) seedlings catalyzed the oxidation of 4-thiouridine, a sulfur-containing nucleoside, to 4-thiouridine disulfide in the presence of H_2O_2. 4-Thiouridine-metabolites such as 4-thiouracil, 4-thio-UMP, 4-thio-UDP, 4-thio-UTP and 4-thio-UDP glucose also were the substrate of the peroxidase reaction. Acidic peroxidase isoenzymes were mainly responsible for the 4-thiouridine oxidation. An increased 4-thiouridine-oxidase activity in the seedlings germinated and grown with the nucleoside was observed. These result suggest that the oxidation of 4-thiouridine and of its metabolites by peroxidase is not beyond the bounds of possibility in 4-thiouridine-cultured radish seedlings.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
19
Start Page
65
End Page
70
ISSN
0370940X
Published Date
1985-12-20
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Life and Environmental Science
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