| File | |
| Title |
ダイコン幼植物体における4-チオウリジン代謝生成物のペルオキシダーゼによる酸化
|
| Title |
Peroxidase-Catalyzed Oxidation of 4-Thiouridine-Metabolites in Radish Seedlings
|
| Title Transcription |
ダイコン ヨウショク ブッタイ ニオケル 4チオウリジン タイシャ セイセイブツ ノ ペルオキシダーゼ ニヨル サンカ
|
| Creator |
Ochiai Hideo
|
| Source Title |
島根大学農学部研究報告
Bulletin of the Faculty of Agriculture, Shimane University
|
| Volume | 19 |
| Start Page | 65 |
| End Page | 70 |
| Journal Identifire |
ISSN 0370940X
|
| Descriptions |
Abstract
Peroxidase (1.11.1.7) purified from Japanese-radish (Raphanus sativus) seedlings catalyzed the oxidation of 4-thiouridine, a sulfur-containing nucleoside, to 4-thiouridine disulfide in the presence of H_2O_2. 4-Thiouridine-metabolites such as 4-thiouracil, 4-thio-UMP, 4-thio-UDP, 4-thio-UTP and 4-thio-UDP glucose also were the substrate of the peroxidase reaction. Acidic peroxidase isoenzymes were mainly responsible for the 4-thiouridine oxidation. An increased 4-thiouridine-oxidase activity in the seedlings germinated and grown with the nucleoside was observed. These result suggest that the oxidation of 4-thiouridine and of its metabolites by peroxidase is not beyond the bounds of possibility in 4-thiouridine-cultured radish seedlings.
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学農学部
Shimane University, Faculty of Agriculture
|
| Date of Issued | 1985-12-20 |
| Access Rights | open access |
| Relation |
[NCID]
AN00108015
|
