The habitat choice of population in spider,Agelenna limbata Thorell, was investigated in the expenmental farm of Shimane University. There were more individuals inhabited in box-tree(Ilex crenata Thumb.) than those lived in the trees of cherry or azalea (Rhododendeon indicum). The box-tree generally has many twigs, therefore, it is assumed that the tree form of box-tree would facilitate a making nest by spiders and a escaping an attack of bird. The population density of spider per hill of box-tree showed an aggregated distribution pattern.The food resources for spiders were wealthy in the box-tree which had high population density of spider.