On inducing artificial mutation with ethylene-imine, the effect of inner and outer factors, differed in degree, had been studied, using rice, wheat and barley.
1. Poor water content(ca.9%) of seeds after dry storage or high temperature(30℃) of treatment showed the decrease of germinating ability by the soaking treatment of ethylene-imine. And the former had rather more influence in rice, the latter in wheat and barley.
2. The intensity of treatment, concentration of solution×treated hours, were to decide germination percentage relatively. Then high(30℃) or low(10℃) temperare made another definite series in their relationships.
3. Various kinds of chlorophyll variation of M_1 seedlings appeared more under the treatments of drier seeds, higher temperature, higher concentration and longer hours.