Faculty of Agriculture, Shimane University
ISSN:0370-940X
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Use this link to cite this item : https://ir.lib.shimane-u.ac.jp/1977
Bulletin of the Faculty of Agriculture, Shimane University 24
1990-12-21 発行
馬鈴薯ガラクトリパーゼ処理による葉緑体膜からのフェレドキシン : NADP還元酵素ならびにプラストシアニンの脱離
Release of Ferredoxin-NADP Reductase and Plastocyanin from Spinach Chloroplast Membranes by Potato Galactolipase
Hirayama, Osamu
Nomotobori, Takashi
Sumi, Haruo
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Description
Spinach class II chloroplasts were trearted with purified potato galactolipase, and the effects on the photoactivities and the functional proteins were investigated.
A treatment of chloroplasts with the enzyme in the absence of bovine serum albumin caused a marked decrease in NADP photoreduction and a great increase in NADPH-diaphorase activity. This indicates that the ferredoxin-NADP reductase was released from the membranes by the enzyme action. The rate of the release by the lipolytic enzyme was much higher than those of release by sonication with or without Triton X-100. Other treatment with the enzyme in the presence of bovine serum albumin, followed by a separation by density gradient centrifugation, also caused a decrease in photosystem I activity, but the decrease was recovered with addition of plastocyanin. This indicates a release of plastocyanin by the enzyme.
A treatment of chloroplasts with the enzyme in the absence of bovine serum albumin caused a marked decrease in NADP photoreduction and a great increase in NADPH-diaphorase activity. This indicates that the ferredoxin-NADP reductase was released from the membranes by the enzyme action. The rate of the release by the lipolytic enzyme was much higher than those of release by sonication with or without Triton X-100. Other treatment with the enzyme in the presence of bovine serum albumin, followed by a separation by density gradient centrifugation, also caused a decrease in photosystem I activity, but the decrease was recovered with addition of plastocyanin. This indicates a release of plastocyanin by the enzyme.
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