Bulletin of the Faculty of Agriculture, Shimane University

Faculty of Agriculture, Shimane University

ISSN:0370-940X

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Bulletin of the Faculty of Agriculture, Shimane University 23

1989-12-21 発行

糸状性,好温性ラン藻Phormidium lapideumの制限酵素

Restriction Endonucleases from Phormidium lapideum, a Strain of Filamentous and Thermophilic Cyanobacteria

Ochiai, Hideo

Shibata, Hitoshi

Sawa, Yoshihiro

Ashida, Naomi

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d0030023n029.pdf 1.95 MB

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A couple of restriction endonucleases, Pla I and Pla II, have been purified from a filametous and thermophilic cyanobacterium, Phormidium lapideum. Pla I was proved to be an isoschizomer of Hae III to cleavage the site GG↓CC and was a monomeric protein that had a molecular weight of about 40 kilodaltons. Pla II was an isoschizomer of Nsp (7524) V to recognize the site TTCGAA and was estimated as a heterotetrameric protein (α_2β_2). Pla II has an apparent molecular mass of 176 kilodaltons and that of α subunit was 63 kilodaltons, β subunit 31 kilodaltons. Characteristics of Pla I and Pla II were investigated in comparison with that of the respective isoschizomers, Hae III and Nsp (7524) V.

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d0030023n029.pdf 1.95 MB

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