Bulletin of the Faculty of Agriculture, Shimane University

Faculty of Agriculture, Shimane University

ISSN:0370-940X

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Use this link to cite this item : https://ir.lib.shimane-u.ac.jp/1661

Bulletin of the Faculty of Agriculture, Shimane University 13

1979-12-15 発行

硝酸塩還元性細菌の簡便識別法

A Rapid Method for the Differentiation of Nitrate Reducer by Reagent-Agar Overlay Technique

Matsumoto, Muneto

Nishitai, Yujiro

Kayashima, Takayuki

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d0030013n017.pdf 3.97 MB

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A rapid plate method is described for the differentiation of nitrate reducer in lieu of the conventional liquid culture method. The method includes an ordinary plate culture with bouillon-agar, subsequent treatment with overlaying agar containing nitrate and Griss-Romin reagent on the plate culture, and counting of red colonies developed. With a considerable seving of time, trouble, and culture media, easy estimation of the population ratio of nitrate reducer in the natural environment was attained by this method. By modifying the pH value of the original Griss Romin reagent from 2.2 to 4.0, more viable cells were able to be picked up with less reagent injury from the plate after the differentiation test.

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d0030013n017.pdf 3.97 MB

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30周年記念号発刊によせて

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