In tomato culture under protected cultivation, it is important subject to enhance planting density in raising seedling stage. In the present experiment, foliar-sprays of plant growth regulators (2.5 ppm uniconazol, 100 ppm paclobutrazol and 1000 ppm chlorocholine chloride), grafting and root pruning treatments were conducted at true-two-leafe stage. In result, it was clarified that using 100 ppm paclobutrazol treatment extremely decreased maximum leaf length. Thus, this treatment could effectively enhance planting density.