| Title Transcription | タジュウ コテイカホウ ニヨッテ チョウセイシタ コテイカ チアミナーゼ 1 ノ コウソガクテキ セイシツ
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| Title Alternative (English) | The Enzymological Properties of Immobilized Thiaminase I Prepared by Means of Multi-Immobilization Method
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| File | |
| language |
eng
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| Author |
Mochida, Kazuo
Ozoe, Yoshihisa
Nakamura, Toshiie
Yokouchi, Takayuki
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| Description | Thiaminase I (EC 2.5.1.2) was immobilized by the combined use of Sepharose 4B, glutaraldehyde, and K-carrageenan in order to enhance its utility. The native enzyme was inactivated by the addition of organic solvents into an aqueous solution in order to promote the solubility of apolar substrates. However, the multi-immobilized enzymes were not only tolerant to organic solvents but also stable to heat. In addition, the activities of the immobilized enzymes increased during repeated utilizations. Thus, the utility of thiaminase I as a catalyst may be greatly enhanced by the multi-immobilization.
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| Journal Title |
Bulletin of the Faculty of Agriculture, Shimane University
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| Volume | 21
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| Start Page | 178
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| End Page | 187
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| ISSN | 0370940X
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| Published Date | 1987-12-21
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| NCID | AN00108015
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| Publisher | 島根大学農学部
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| Publisher Aalternative | Shimane University, Faculty of Agriculture
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| NII Type |
Departmental Bulletin Paper
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| OAI-PMH Set |
Faculty of Life and Environmental Science
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| 他の一覧 |
