| Title Transcription | ヨウリョクタイ ノ シシツ ブンカイ コウソ ショリ ナラビニ ダツ シシツ ヨウリョクタイ ノ トクセイ
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| Title Alternative (English) | Lipolytic Enzyme Treatment of chloroplasts and Characterization of Lipid-Depleted Chloroplasts
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| File | |
| language |
eng
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| Author |
Hirayama, Osamu
Nomotobori, Takashi
Morita, Kohkichi
Okuda, Toshihiko
Ichihara, Minoru
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| Description | Potato galactolipase treatment of spinach chloroplasts were investigated. The enzyme reaction was inhibited with Mg<Cl>___2, bovine serum albumin and sucrose, to varying levels with their specific action on the chloroplast grana stacking. The reaction increased in three steps with increase of temperature, suggesting that there are different localization and existing forms of lipids. By the enzyme treatment and subsequent centrifugation on density-gradient column, lipid-depleted chloroplasts were prepared. Analysis revealed that the preparation retained nearly half portion of the intial electron flows, but H^+ uptake and phosphorylation activities were completely lost.
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| Journal Title |
Bulletin of the Faculty of Agriculture, Shimane University
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| Volume | 15
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| Start Page | 56
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| End Page | 60
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| ISSN | 0370940X
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| Published Date | 1981-12-15
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| NCID | AN00108015
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| Publisher | 島根大学農学部
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| Publisher Aalternative | Shimane University, Faculty of Agriculture
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| NII Type |
Departmental Bulletin Paper
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| OAI-PMH Set |
Faculty of Life and Environmental Science
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| 他の一覧 |
