| File | |
| language |
eng
|
| Author |
Yoshimatsu, K
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843, Japan.
Kawahara, N
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843, Japan.
Yamamoto, S.-I.
National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602, Japan.
Niino, T.
Gene Research Center, University of Tsukuba, Tsukuba 305-8572, Japan.
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| Description | A clonal propagation method by node culture of perilla (Perilla frutescens L. Britton) was investigated. Nodes were plated on solidified 1/2 MS medium with BA and optimum shoot elongation and propagation was obtained at BA 0.05 and 0.1 mg l-1. Cryopreservation using an aluminum cryo-plate was successfully applied to in vitro-grown perilla shoot tips. Excised shoot tips from nodes were precultured on 1/2 MS medium with 0.3 M sucrose and embedded on an aluminum cryo-plate with alginate gel. The cryo-plate with shoot tips was osmo-protected with LS solution and dehydrated in PVS2 for 20 min at 25°C prior to immersion into liquid nitrogen. The recovery growth after cryopreservation was found to be about 80%. This new V-Cryo-plate method has many advantages and may facilitate the cryo-storage of other medicinal plants.
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| Subject | Cryopreservation
Aluminum
Regrowth
Vitrification
Plants
Clonal propagation
Hypocotyls
Chromosomes
Plant genetics
Japanese culture
|
| Journal Title |
Advances in Horticultural Science
|
| Volume | 28
|
| Issue | 2
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| Start Page | 79
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| End Page | 83
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| Published Date | 2014
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| DOI | |
| Publisher | Dipartimento Di Scienze Delle Produzioni Vegetali, Del Suolo E Dell'Ambiente Agroforestale – DiPSA – University of Florence
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| NII Type |
Journal Article
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| Format |
PDF
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| Relation | |
| Text Version |
出版社版
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| Gyoseki ID | e26030
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| OAI-PMH Set |
Faculty of Life and Environmental Science
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