| ファイル情報(添付) | |
| タイトル |
Development of in vitro propagation by node culture and cryopreservation by V-Cryo-plate method for Perilla frutescens
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| 著者 |
Yoshimatsu K
Kawahara N
Yamamoto S.-I.
Niino T.
|
| 収録物名 |
Advances in Horticultural Science
|
| 巻 | 28 |
| 号 | 2 |
| 開始ページ | 79 |
| 終了ページ | 83 |
| 内容記述 |
その他
A clonal propagation method by node culture of perilla (Perilla frutescens L. Britton) was investigated. Nodes were plated on solidified 1/2 MS medium with BA and optimum shoot elongation and propagation was obtained at BA 0.05 and 0.1 mg l-1. Cryopreservation using an aluminum cryo-plate was successfully applied to in vitro-grown perilla shoot tips. Excised shoot tips from nodes were precultured on 1/2 MS medium with 0.3 M sucrose and embedded on an aluminum cryo-plate with alginate gel. The cryo-plate with shoot tips was osmo-protected with LS solution and dehydrated in PVS2 for 20 min at 25°C prior to immersion into liquid nitrogen. The recovery growth after cryopreservation was found to be about 80%. This new V-Cryo-plate method has many advantages and may facilitate the cryo-storage of other medicinal plants.
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| 主題 |
Cryopreservation
Aluminum
Regrowth
Vitrification
Plants
Clonal propagation
Hypocotyls
Chromosomes
Plant genetics
Japanese culture
|
| 言語 |
英語
|
| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Dipartimento Di Scienze Delle Produzioni Vegetali, Del Suolo E Dell'Ambiente Agroforestale – DiPSA – University of Florence
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| 発行日 | 2014 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 関連情報 |
[DOI] 10.2307/24586801
[URI] https://www.jstor.com/stable/24586801
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