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Title Transcription
ヨウリョクタイ ノ シシツ ブンカイ コウソ ショリ ナラビニ ダツ シシツ ヨウリョクタイ ノ トクセイ
Title Alternative (English)
Lipolytic Enzyme Treatment of chloroplasts and Characterization of Lipid-Depleted Chloroplasts
File
language
eng
Author
Hirayama, Osamu
Nomotobori, Takashi
Morita, Kohkichi
Okuda, Toshihiko
Ichihara, Minoru
Description
Potato galactolipase treatment of spinach chloroplasts were investigated. The enzyme reaction was inhibited with Mg<Cl>___2, bovine serum albumin and sucrose, to varying levels with their specific action on the chloroplast grana stacking. The reaction increased in three steps with increase of temperature, suggesting that there are different localization and existing forms of lipids. By the enzyme treatment and subsequent centrifugation on density-gradient column, lipid-depleted chloroplasts were prepared. Analysis revealed that the preparation retained nearly half portion of the intial electron flows, but H^+ uptake and phosphorylation activities were completely lost.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
15
Start Page
56
End Page
60
ISSN
0370940X
Published Date
1981-12-15
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Life and Environmental Science
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