Title Transcription
タジュウ コテイカホウ ニヨッテ チョウセイシタ コテイカ チアミナーゼ 1 ノ コウソガクテキ セイシツ
Title Alternative
The Enzymological Properties of Immobilized Thiaminase I Prepared by Means of Multi-Immobilization Method
File
language
eng
Author
Mochida, Kazuo
Ozoe, Yoshihisa
Nakamura, Toshiie
Yokouchi, Takayuki
Description
Thiaminase I (EC 2.5.1.2) was immobilized by the combined use of Sepharose 4B, glutaraldehyde, and K-carrageenan in order to enhance its utility. The native enzyme was inactivated by the addition of organic solvents into an aqueous solution in order to promote the solubility of apolar substrates. However, the multi-immobilized enzymes were not only tolerant to organic solvents but also stable to heat. In addition, the activities of the immobilized enzymes increased during repeated utilizations. Thus, the utility of thiaminase I as a catalyst may be greatly enhanced by the multi-immobilization.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
21
Start Page
178
End Page
187
ISSN
0370940X
Published Date
1987-12-21
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Life and Environmental Science