Effective utilization of agricultural wastes derived from local products is an important issue. In this study, we evaluated the potential activities of the lateral shoot extract of ‘Shinku,’ a new red grapes cultivar in Shimane Prefecture, which are discarded during cultivation. To utilize the extract in skin care cosmetics, DPPH radical scavenging activity and the inhibition of tyrosinase activity were determined. The relative DPPH radical scavenging activity to total polyphenol concentration of the lateral shoot extract were higher than those of the pruned cane and the fruit extracts. There were little differences among different grape cultivars, Shinku, Shine Muscat and Delaware, in the radical scavenging activity of their lateral shoot extracts. The extracts also showed the inhibitory effect against mushroom-derived tyrosinase activity with L-tyrosine and L-DOPA as substrates. The extract of Shinku showed the highest inhibitory effects among three cultivars. Quercetin was detected as its glycosides in the extracts and assumed as active antioxidant compounds. These results indicate that the lateral shoot extracts of Shinku and other grape cultivars are promising antioxidant materials.