Previously, the writers isolated Cephalosporium sp. from cryptomeria affected by the so-called "Hachikami" disease. During 1959-1960, this fungus was inoculated to sound trunks of cryptomeria by boring or by stripping the bark. On sixth month after inoculation, similar staining just as naturally occured browning was recognized around inoculated and non-inoculated parts.
This fungus grows and germinates at rather high temperature (Opt. temp. 28℃) and in wide range of hydrogen-ion concentration (Opt. pH 4.0).
From the histo-chemical observation, cellulose and lignin contents in the browned tissue seem to be same as sound tissue. These results may correspond with the non-siginficant difference of the compressive strength between the browning and sound wood. The phenol substances increase at browning portion of wood affected by the disease, and present fungus has enzyme which oxidize some phenol compounds. These facts, may be concerned with the occurence of browning on affected wood.