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Title Transcription
トウチョウ バイヨウ ニ ヨリ エタ イチゴ ノ in vitro ニ オケル カイカ ト ケツジツ
Title Alternative (English)
In vitro Flowering and Fruiting of Strawberry through Shoot Apex Culture
Author
Ohtani, Noriyuki Faculty of Life and Environmental Science, Shimane University
Endo, Keita Faculty of Life and Environmental Science, Shimane University
Hosoki, Takashi
Description
茎頂培養により得たイチゴのin vitroにおける開花および結実の方法について検討した. イチゴを300mlフラスコで培養すると, クラウン径は3mm以上大きくならなかった. 一方, カルチャーボトルでイチゴを培養すると, クラウン径が5mmまで大きくなった.
一季成り性品種'とよのか'および四季成り性品種'サマーベリー'ともクラウン径約3mmでは出蕾しなかったが, クラウン径約5mmでは出蕾および開花した. 出蕾率は'とよのか'の場合22.2%, 'サマーベリーの場合84.2%であった. 開花率は'とよのか'の場合11.1%, 'サマーベリー'の場合36.8%であった.
以上より, カルチャーボトルを用いることでin vitroにおけるイチゴの茎頂培養からの開花が可能になり,人工授粉によって果実の結実もみられた.
Description Alternative
Abstract
A method of inducing in vitro flowering and fruiting of strawberry plants obtained by shoot apex culture was investigated. When strawberry plants were cultured in the flask (300 ml), the crown did not enlarge over 3 mm diameter, whereas when cultured in a culture-bottle (100mm × 110mm × 100mm), the crown enlarged to 5 mm diameter.
A June-bearing type strawberry, 'Toyonoka', and an ever-bearing type, 'Summerberry' with 3 mm crown diameters did not differentiate flower buds, whereas those with 5 mm crown diameters were induced to flowere.
The percentages of flower buds differentiated by 'Toyonoka' and 'Summerberry' crowns were 22.2 % and 84.2 %, respectively, whereas only 11.1 % and 36.8 %, respectively, flowered.
Thus, culture-bottle system enabled in vitro flowering of strawberry. Using this system, fruits were obtained by artificial pollination.
Journal Title
Journal of the Japanese Society for Horticultural Science
Volume
66
Issue
2
Start Page
419
End Page
421
ISSN
0013-7626
ISSN(Online)
1880-358X
Published Date
1997
DOI
Publisher
園芸学会
Publisher Transcription
エンゲイ ガッカイ
Publisher Aalternative
Japanese Society for Horticultural Science
NII Type
Journal Article
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Life and Environmental Science