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language
eng
Author
Xia Da Department of Dermatology, Faculty of Medicine, Shimane University, Izumo, 693-8501, Japan
Nishiyama, Yayoi Institute of Medical Mycology, Teikyo University, Tokyo, 192-0395, Japan
Duerna Tie Department of Dermatology, Faculty of Medicine, Shimane University, Izumo, 693-8501, Japan
Kyaw Zaw Hein Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, 55905, USA
Yamamoto, Osamu Division of Dermatology, Department of Medicine of Sensory and Motor Organs, Faculty of Medicine, Tottori University, Yonago, 683-8503, Japan
Description
Ou-gon, an extract from Scutellaria baicalensis Georgi root, has been shown to exhibit pronounced antifungal activity. The present study aimed to identify antifungal components of Ou-gon and to determine their mechanism of action against pathogenic fungi. Antifungal activity was assessed by the microbroth dilution method using four common human pathogenic fungi, Trichophyton rubrum, Trichophyton mentagrophytes, Aspergillus fumigatus, and Candida albicans. Components of crude Ou-gon extract were separated by reversed-phase high-performance liquid chromatography. Active antifungal components were identified by liquid chromatography-electrospray ionization tandem mass spectrometry. Terminal deoxynucleotidyl transferase dUTP nick end-labelling assay, SYTOX® green uptake assay, determination of intracellular reactive oxygen species and mitochondrial membrane potential as well as microscopy (confocal laser microscopy, scanning and transmission electron microscopy) were used to probe the mode of action. Two components with potent antifungal activity, baicalein and wogonin, were identified in Ou-gon. Baicalein showed potent antifungal activity against the four fungi tested. Wogonin displayed antifungal activity against all four fungi except C. albicans. The components are considered to induce apoptosis-like programmed cell death via hyperproduction of reactive oxygen species. This study enhances our understanding of the antifungal activity of Kampo medicine, and may contribute to the development of new and safe antifungal therapeutics.
Journal Title
Scientific Reports
Volume
9
Start Page
Article number: 1683
ISSN(Online)
2045-2322
Published Date
2019-02-08
DOI
Publisher
Springer Nature
NII Type
Journal Article
Format
PDF
Relation
Text Version
出版社版
Gyoseki ID
e38195
OAI-PMH Set
Faculty of Medicine