Antifungal activity and mechanism of action of Ou-gon (Scutellaria root extract) components against pathogenic fungi

Scientific Reports 9 巻 Article number: 1683- 頁 2019-02-08 発行
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ファイル情報(添付)
タイトル
Antifungal activity and mechanism of action of Ou-gon (Scutellaria root extract) components against pathogenic fungi
著者
Xia Da
Nishiyama Yayoi
Duerna Tie
Kyaw Zaw Hein
Yamamoto Osamu
収録物名
Scientific Reports
9
開始ページ Article number: 1683
収録物識別子
EISSN 2045-2322
内容記述
その他
Ou-gon, an extract from Scutellaria baicalensis Georgi root, has been shown to exhibit pronounced antifungal activity. The present study aimed to identify antifungal components of Ou-gon and to determine their mechanism of action against pathogenic fungi. Antifungal activity was assessed by the microbroth dilution method using four common human pathogenic fungi, Trichophyton rubrum, Trichophyton mentagrophytes, Aspergillus fumigatus, and Candida albicans. Components of crude Ou-gon extract were separated by reversed-phase high-performance liquid chromatography. Active antifungal components were identified by liquid chromatography-electrospray ionization tandem mass spectrometry. Terminal deoxynucleotidyl transferase dUTP nick end-labelling assay, SYTOX® green uptake assay, determination of intracellular reactive oxygen species and mitochondrial membrane potential as well as microscopy (confocal laser microscopy, scanning and transmission electron microscopy) were used to probe the mode of action. Two components with potent antifungal activity, baicalein and wogonin, were identified in Ou-gon. Baicalein showed potent antifungal activity against the four fungi tested. Wogonin displayed antifungal activity against all four fungi except C. albicans. The components are considered to induce apoptosis-like programmed cell death via hyperproduction of reactive oxygen species. This study enhances our understanding of the antifungal activity of Kampo medicine, and may contribute to the development of new and safe antifungal therapeutics.
言語
英語
資源タイプ 学術雑誌論文
出版者
Springer Nature
発行日 2019-02-08
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
関連情報
[DOI] 10.1038/s41598-019-38916-w
[URI] https://www.nature.com/srep/