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Title Transcription
ダイチョウキン K-12 カブ L-ガタ ノ ブンリ ト ソノ セイシツ
Title Alternative
Isolation and its characters of Escherichia coli K-12, L-form
File
language
eng
Author
Onoda, Tetsuo
Description
Suspension of Escherichia coli K-12 was sequentially treated with N-methyl-N-nitro-N-nitrosoguanidine and lysozyme,and its L-forms were selected on brain heart infusion (BHI) agar medium. Of those clones,L-form NC7 was adapted to grow in a liquid cultue with osmotic stabilizer. The L-form NC7 was stable in penicillin-free medium. Morphology of this L-form NC7 agreed with that described for other bacterial L-forms in many respects. The L-form NC7 multiplied in soft agar (0.5%, W/V), but did not in hard agar (1.5%, W/V). In osmotic stabilizers tested,NaCl was most effective in the range of 2 to 3% (W/V). In BHI medium containing NaCl CaCl_2 was required for growth of the L-form NC7. Studies of the growth characteristics for L-form NC7 gave the following results:an optimal temperature of 32℃, an optimal pH of 7 to 8,and no requirement for serum . This L-form NC7 also retained many of the physiological characters of the parent strain.
Journal Title
Memoirs of the Faculty of Science, Shimane University
Volume
20
Start Page
69
End Page
76
ISSN
03879925
Published Date
1986-12-25
NCID
AN00108106
Publisher
島根大学理学部
Publisher Aalternative
The Faculty of Science, Shimane University
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Science and Engineering