タジュウ コテイカホウ ニヨッテ チョウセイシタ コテイカ チアミナーゼ 1 ノ コウソガクテキ セイシツ
The Enzymological Properties of Immobilized Thiaminase I Prepared by Means of Multi-Immobilization Method
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Thiaminase I (EC 126.96.36.199) was immobilized by the combined use of Sepharose 4B, glutaraldehyde, and K-carrageenan in order to enhance its utility. The native enzyme was inactivated by the addition of organic solvents into an aqueous solution in order to promote the solubility of apolar substrates. However, the multi-immobilized enzymes were not only tolerant to organic solvents but also stable to heat. In addition, the activities of the immobilized enzymes increased during repeated utilizations. Thus, the utility of thiaminase I as a catalyst may be greatly enhanced by the multi-immobilization.
Bulletin of the Faculty of Agriculture, Shimane University
Shimane University, Faculty of Agriculture
Departmental Bulletin Paper
Faculty of Life and Environmental Science