File
language
eng
Author
Okunishi, Hideki
Wang, Dan-Qiao
Kakizoe, Eiichi
Shimoura, Keiko
Description
We introduce an improved perfusion technique that allows sensitive detection of vasomotion as well as differential estimation of drug action upon luminal or abluminal application. The technique employs a short, isolated segment of medium-sized artery (internal diameter 0.7-4.0mm) that would not generate sufficient resistance by conventional technique. By our method, the constant flow goes through a narrow gap between the intimal surface of the vessel and the outer wall of the blind-end tubing that occupies a large dead-space in the vascular lumen. Due to this narrow clearance, subtle vasomotion can be amplified onto the perfusion pressure, while not much flow-rate is necessary to maintain the baseline perfusion pressure. The method also allows application of drugs either from intimal or adventitial side. This technique should provide powerful means for physiological and pharmacological studies on blood vessels.
Journal Title
Shimane journal of medical science
Volume
18
Issue
1
Start Page
11
End Page
20
ISSN
03865959
ISSN(Online)
24332410
Published Date
2000-06-01
NCID
AA00841586
Publisher Aalternative
Shimane Medical University
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
Remark
http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html