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language
eng
Author
ChoongSoo Yun Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan
Amakata, Daiki Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan
Matsuda, Hideyuki
Description
The betaproteobacterium Mitsuaria chitosanitabida (formerly Matsuebacter chitosanotabidus) 3001 produces a chitosanase (ChoA) that is classified in glycosyl hydrolase family 80. While many chitosanase genes have been isolated from various bacteria to date, they show limited homology to the M. chitosanitabida 3001 chitosanase gene (choA). To investigate the phylogenetic distribution of chitosanases analogous to ChoA in nature, we identified 67 chitosan-degrading strains by screening and investigated their physiological and biological characteristics. We then searched for similarities to ChoA by Western blotting and Southern hybridization and selected 11 strains whose chitosanases showed the most similarity to ChoA. PCR amplification and sequencing of the chitosanase genes from these strains revealed high deduced amino acid sequence similarities to ChoA ranging from 77% to 99%. Analysis of the 16S rRNA gene sequences of the 11 selected strains indicated that they are widely distributed in the β and γ subclasses of Proteobacteria and the Flavobacterium group. These observations suggest that the ChoA-like chitosanases that belong to family 80 occur widely in a broad variety of bacteria.
Subject
Betaproteobacteria
Chitosan
Flavobacterium
Gammaproteobacteria
glycoside hydrolases
Journal Title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume
71
Issue
9
Start Page
5138
End Page
5144
ISSN
0099-2240
ISSN(Online)
1098-5336
Published Date
2005-09
DOI
Publisher
American Society for Microbiology
NII Type
Journal Article
Format
PDF
Text Version
著者版
OAI-PMH Set
Faculty of Life and Environmental Science