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eng
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Original article
Author
GONG, AO
Description
Qualitative and quantitative changes in collagens are linked to various diseases. Monitoring of collagens may provide new insights into the assessment and diagnosis of collagen-related diseases. In this study, we established a method that enables simultaneous quantification of the α chains of types I, III, and V collagens, namely COL1A1, COL3A1, and COL5A1, in a culture medium of LX-2 human hepatic stellate cells using nano-liquid chromatography tandem mass spectrometry (nano-LC/MS/MS). Specifically, simultaneous quantification of multiple collagen α chains could be accomplished by using their specific target peptides. Compared with conventional enzyme-linked immunosorbent assay (ELISA), this method is more sensitive and efficient for simultaneous quantification of various collagens. These data suggested that our newly developed quantification method is valuable and could be used for the diagnosis of collagen-related diseases.
Subject
collagens
nano-LC/MS/MS
LX-2 cells
EDS
ELISA
Journal Title
Shimane Journal of Medical Science
Volume
40
Issue
1-2
Start Page
9
End Page
21
ISSN
03865959
ISSN(Online)
24332410
Published Date
2023-06
NCID
AA00841586
DOI
Publisher
Faculty of Medicine, Shimane University
Publisher Aalternative
島根大学医学部
NII Type
Departmental Bulletin Paper
Format
PDF
Rights
Faculty of Medicine, Shimane University
rights(link)
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
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