| Title Transcription | ダイコン シヨウ ノ ヨウリョクタイ ハッセイ ニ カンスル ケンキュウ 4 リンゴサン ダツ スイソ コウソ ニツイテ
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| Title Alternative (English) | Studies on the Chloroplast Development in Radish Cotyledons(4) : on Malate Dehydrogenases
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| File | |
| language |
jpn
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| Author |
Ochiai, Hideo
Kono, Yasuhisa
Shibata, Hitoshi
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| Description | ocalization, properties and time courses of malate dehydrogenases in greening radish cotyledons were studied as well as an inhibitory effect of 4-thiouridine upon the enzymes. Results obtained are as follow.
1) Chloroplast fraction isolated from the radish cotyledon cells contained <NAD>^+ specific malate dehydrogenase, the specific activity of which decreased as the development of the chloroplast proceeded on illumination. 2) Non-chloroplast fraction contained <NADP>^+-malate dehydrogenase and malic enzyme. Cofactor of the <NADP>^+_malate dehydrogenase could be replaced by <NAD>^+ with a slight decrease of the specific activity. Malic enzyme required <NADP>^+ as a specific cofactor. Specific activities of both the enzymes remained unchanged with illumination time. 3) <NAD>^+_malate dehydrogenase of the chloroplast was inhibited by the presence of 4-thiouridine (1-5mM), but malic enzyme in the cytoplasm was not. Based upon these observations, biological significance of <NAD>^+_malate dehydrogenase in the developing chloroplast was discussed. |
| Journal Title |
Bulletin of the Faculty of Agriculture, Shimane University
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| Volume | 7
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| Start Page | 129
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| End Page | 134
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| ISSN | 0370940X
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| Published Date | 1973-12-15
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| NCID | AN00108015
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| Publisher | 島根大学農学部
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| Publisher Aalternative | Shimane University, Faculty of Agriculture
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| NII Type |
Departmental Bulletin Paper
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| OAI-PMH Set |
Faculty of Life and Environmental Science
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| 他の一覧 |
