Author |
Qing, YAN
Department of Developmental Biology
Ogawa, Noriko
Department of Developmental Biology
Matsumoto, Akihiro
Department of Developmen
Yuqi, DANG
The Traditional Chinese Medicine Hospital of Yinchuan
Otani, Hiroki
Department of Developmen
|
Description | Effects of Kampo formulas which have been long
used clinically for obese patients remain unclear at the cellular level. The objective of this study is to investigate the effects of 8 commonly-used Kampos on the differentiation and proinflammatory cytokines expression of 3T3-L1 cells. 3T3-L1 preadipocytes were induced differentiation being treated with test 8 Kampos through the induction period. Oil red O staining and mRNAs expressions of peroxisome proliferator- active receptor-γ (PPARγ) and adiponectin were examined to evaluate effects of the Kampos on the differentiation. Tumor necrosis factor-alpha (TNF-α), interleukin-6, and monocyte chemoattractant protein-1 (MCP-1) mRNAs were examined to investigate the anti-inflammatory effects of the Kampos. PPARγ and/or adiponectin, as well as MCP-1 and/or TNF-α mRNAs were significantly decreased by Boiogito and a few other Kampos when compared with the control. These results at the cellular level are compatible with the clinical beneficial effects of these Kampos. |
Subject | 3T3-L1
differentiation
inflammation
PPARγ
MCP-1
|
Journal information |
Shimane Journal of Medical Science
34
( 3
), 109
- 118
, 2018-03-31
|
Publisher | Faculty of Medicine, Shimane University
|
Rights | Faculty of Medicine, Shimane University
|