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language
eng
Author
Moriyama, Daisuke QOL division, Kaneka Corporation, Takasago, Japan
Hosono, Kouji Faculty of Life and Environmental Science, Department of Life Science and Biotechnology, Shimane University, Matsue, Japan
Fujii, Makoto Faculty of Life and Environmental Science, Department of Life Science and Biotechnology, Shimane University, Matsue, Japan
Washida, Motohisa QOL division, Kaneka Corporation, Takasago, Japan
Nanba, Hirokazu QOL division, Kaneka Corporation, Takasago, Japan
Kaino, Tomohiro Faculty of Life and Environmental Science, Department of Life Science and Biotechnology, Shimane University, Matsue, Japan
Description
Coenzyme Q10 (CoQ10) is essential for energy production and has become a popular supplement in recent years. In this study, CoQ10 productivity was improved in the fission yeast Schizosaccharomyces pombe. Ten CoQ biosynthetic genes were cloned and overexpressed in S. pombe. Strains expressing individual CoQ biosynthetic genes did not produce higher than a 10% increase in CoQ10 production. In addition, simultaneous expression of all ten coq genes did not result in yield improvements. Genes responsible for the biosynthesis of p-hydroxybenzoate and decaprenyl diphosphate, both of which are CoQ biosynthesis precursors, were also overexpressed. CoQ10 production was increased by overexpression of Eco_ubiC (encoding chorismate lyase), Eco_aroFFBR (encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase) or Sce_thmgr1 (encoding truncated HMG-CoA reductase). Furthermore, simultaneous expression of these precursor genes resulted in 2-fold increases in CoQ10 production
Subject
Coenzyme Q
ubiquinone
S. pombe
isoprenoids
mevalonate
Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume
79
Issue
796
Start Page
1026
End Page
1033
ISSN
0916-8451
ISSN(Online)
1347-6947
Published Date
2015-2-3
DOI
Publisher
Tokyo, Japan : Japan Society for Bioscience, Biotechnology, and Agrochemistry, c1992-
NII Type
Journal Article
Format
PDF
Text Version
著者版
OAI-PMH Set
Faculty of Life and Environmental Science
Remark
This is an electronic version of an article published in Bioscience, Biotechnology, and Biochemistry [Volume79 No.796, pp1026-1033, 2015 ]. Bioscience, Biotechnology, and Biochemistry is available online at: https://www.tandfonline.com/doi/full/10.1080/09168451.2015.1006573
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