Memoirs of the Faculty of Science, Shimane University

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Memoirs of the Faculty of Science, Shimane University 29
1995-12-25 発行

大腸菌・L-型菌NC7からのカルシウム結合性タンパク質

Study on Calcium-Binding Protein from Escherichia coli L-form NC7
Yawata, Toshio
Onoda, Tetsuo
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Description
A calmodulin-like protein (CaLP) that has been regarded as a Ca2+-dependent modulator protein in Escherichia coli L-form NC7 was purified by fluphenazine-sepharose 4B affinity chromatography. The molecular weight of the CaLP was approx. 18,000 kDa on SDS-Polyacrylamide gel electrophoresis and isoelectric point of 4.5 was confirmed by electric focusing gel (pH 3 to 10). The CaLP were heat stable and exhibited Ca2+-binding property. Further, the CaLP stimulated bovine hart phosphodiesterase activity. These results suggest the presence of Ca2+-regulatory system in prokaryotes.