| Title Transcription | ダイチョウキン Lガタキン ノ ゾウショク ニオケル カルシウム ヒ イゾンセイ ヘンイカブ ノ セイシツ ニツイテ
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| Title Alternative (English) | Change in Ca-Requirement on Growth of a Variant, Cig-1, derived from a Stable L-Form of Escherichia coli K-12
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| File | |
| language |
eng
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| Author |
Onoda, Tetsuo
Nishigami, Kazuyoshi
Sakamoto, Yayoi
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| Description | A variant, Cig-1 (calcium independent growth) was selected from L-form NC7 culture belonging to the genus Escherichia coli K-12. The Cig-1 differed from the parental L-form NC7 strain in growth response to calcium content . Growth of the L-form NC7 was markedly inhibited by addition of EGTA (1mM) , while Cig-1 was able to grow in the presence of EGTA. When the Cig-1 was transfered to growth medium containing 10μM Ca ionophore, A 23187, the cells did not grow, but E. coli cells were able to grow under the same conditions. When 10 μM of carbonyl cyanide-m-chlorophenylhydrazone (CCCP), a protonophore, was added to growth medium containing glucose at pH 7.5, growth of the Cig-1 was completely blocked, suggesting that proton motive force is requsired for growth of the Cig-1 strain.
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| Journal Title |
Memoirs of the Faculty of Science, Shimane University
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| Volume | 21
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| Start Page | 97
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| End Page | 103
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| ISSN | 03879925
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| Published Date | 1987-12-25
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| NCID | AN00108106
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| Publisher | 島根大学理学部
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| Publisher Aalternative | The Faculty of Science, Shimane University
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| NII Type |
Departmental Bulletin Paper
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| OAI-PMH Set |
Faculty of Science and Engineering
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| 他の一覧 |
