| File | |
| Title |
大腸菌L-型菌の増殖におけるカルシウム非依存性変異株の性質について
|
| Title |
Change in Ca-Requirement on Growth of a Variant, Cig-1, derived from a Stable L-Form of Escherichia coli K-12
|
| Title Transcription |
ダイチョウキン Lガタキン ノ ゾウショク ニオケル カルシウム ヒ イゾンセイ ヘンイカブ ノ セイシツ ニツイテ
|
| Creator |
Onoda Tetsuo
Nishigami Kazuyoshi
Sakamoto Yayoi
|
| Source Title |
島根大学理学部紀要
Memoirs of the Faculty of Science, Shimane University
|
| Volume | 21 |
| Start Page | 97 |
| End Page | 103 |
| Journal Identifire |
ISSN 03879925
|
| Descriptions |
Abstract
A variant, Cig-1 (calcium independent growth) was selected from L-form NC7 culture belonging to the genus Escherichia coli K-12. The Cig-1 differed from the parental L-form NC7 strain in growth response to calcium content . Growth of the L-form NC7 was markedly inhibited by addition of EGTA (1mM) , while Cig-1 was able to grow in the presence of EGTA. When the Cig-1 was transfered to growth medium containing 10μM Ca ionophore, A 23187, the cells did not grow, but E. coli cells were able to grow under the same conditions. When 10 μM of carbonyl cyanide-m-chlorophenylhydrazone (CCCP), a protonophore, was added to growth medium containing glucose at pH 7.5, growth of the Cig-1 was completely blocked, suggesting that proton motive force is requsired for growth of the Cig-1 strain.
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学理学部
The Faculty of Science, Shimane University
|
| Date of Issued | 1987-12-25 |
| Access Rights | open access |
| Relation |
[NCID]
AN00108106
|
