| File | |
| Title |
多重固定化法によって調製した固定化チアミナーゼ I の酵素学的性質
|
| Title |
The Enzymological Properties of Immobilized Thiaminase I Prepared by Means of Multi-Immobilization Method
|
| Title Transcription |
タジュウ コテイカホウ ニヨッテ チョウセイシタ コテイカ チアミナーゼ 1 ノ コウソガクテキ セイシツ
|
| Creator |
Nakamura Toshiie
Yokouchi Takayuki
|
| Source Title |
島根大学農学部研究報告
Bulletin of the Faculty of Agriculture, Shimane University
|
| Volume | 21 |
| Start Page | 178 |
| End Page | 187 |
| Journal Identifire |
ISSN 0370940X
|
| Descriptions |
Thiaminase I (EC 2.5.1.2) was immobilized by the combined use of Sepharose 4B, glutaraldehyde, and K-carrageenan in order to enhance its utility. The native enzyme was inactivated by the addition of organic solvents into an aqueous solution in order to promote the solubility of apolar substrates. However, the multi-immobilized enzymes were not only tolerant to organic solvents but also stable to heat. In addition, the activities of the immobilized enzymes increased during repeated utilizations. Thus, the utility of thiaminase I as a catalyst may be greatly enhanced by the multi-immobilization.
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学農学部
Shimane University, Faculty of Agriculture
|
| Date of Issued | 1987-12-21 |
| Access Rights | open access |
| Relation |
[NCID] AN00108015
|
