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File
language
eng
Author
Ito, Motohiko
Fukuda Haruo
Suzuki, Yukio
Harada, Takayuki
Morikawa, Shigeru
Description
Immunohistochemical localization of lactate dehydrogenase subunits was studied using a fluorescent antibody technique in porcine liver. Because of the difficulty in demonstrating specific fluorescence of LDH-M in the liver tissue, many fixatives were tested both in frozen and paraffin sections to obtain consistent staining results. Among the fixatives examined, ether-ethanol-acetone mixture in frozen sections gave the best result in demonstrating both subunits in the liver tissue by immunofluorescence. Paraffin sections failed to preserve LDH-M specific fluorescence in this study. Specific fluorescence for LDH-H was observed in the hepatic cell membrane facing the space of Disse, in the lining cells of sinusoids and in the cyto plasm of Kupffer cells. LDH-M specific fluorescence was observed as fine granules in the cytoplasm of hepatic cells.
Subject
LDH subunits
fluorescent antibody
liver
Journal Title
Shimane journal of medical science
Volume
2
Issue
1
Start Page
25
End Page
31
ISSN
03865959
ISSN(Online)
24332410
Published Date
1978-09-01
NCID
AA00841586
Publisher Aalternative
Shimane Medical University
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine