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Title Transcription
ムハイセイショクガタ ベニシダ DRYOPTERIS ERYTHROSORA ホウシ バイヨウ ノ テキセイ バイチ ノ ケントウ
Title Alternative (English)
Examination of an appropriate nutrient medium for spore culture of apogamy fern plant Dryopteris erythrosora
File
language
jpn
Author
Matsuura, Kazue
Description
In the study of the reproduction mechanism of the fern plants, the method to carry out spore culture effectively and stability are required. However, the appropriate nutrient medium composition of the fern spore culture is not shown clearly. In this study, the composition of MS medium, agar and sucrose in different densities were examined comparatively. The most appropriate nutrient medium composition, 3/4MS : 0.7% agar : 0% sucrose was determined for the spore culture of apogamy fern Dryopteris erythrosora. Furthermore, it was confirmed that the nutrient medium of 3% sucrose obstructed the prothallium formation, but promoted the increase of the undifferentiated cells. The method to culture and increase prothalliums of the hybrid spores that had low germination rate was inspected, that 3% sucrose nutrient medium was used to culture and increase protonemas of the hybrid spores, and then, the protonemas were moved to the 0% sucrose nutrient medium for the prothallium culture, the result was that the number of the prothalliums was increased.
Subject
Dryopteris erythrosora
apogamy
prothallium
gametophyte
MS medium
Journal Title
島根大学生物資源科学部研究報告
Volume
18
Start Page
17
End Page
21
ISSN
13433644
Published Date
2013-09-30
NCID
AA1112906X
Publisher
島根大学生物資源科学部
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Life and Environmental Science
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