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language
eng
Title Alternative (English)
Direct exposure to mild heat promotes proliferation and neuronal differentiation of neural stem/progenitor cells in vitro.
Author
Md Emon Hossain
Katakura, Masanori
Sugimoto, Naotoshi Department of Physiology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
Abdullah Al Mamun
Rafiad Islam
Hashimoto, Michio
Description
Heat acclimation in rats is associated with enhanced neurogenesis in thermoregulatory centers of the hypothalamus. To elucidate the mechanisms for heat acclimation, we investigated the effects of direct mild heat exposure on the proliferation and differentiation of neural stem/progenitor cells (NSCs/NPCs). The NSCs/NPCs isolated from forebrain cortices of 14.5-day-old rat fetuses were propagated as neurospheres at either 37.0°C (control) or 38.5°C (mild heat exposure) for four days, and the effects on proliferation were investigated by MTS cell viability assay, measurement of neurosphere diameter, and counting the total number of cells. The mRNA expressions of heat shock proteins (HSPs) and brain-derived neurotrophic factor (BDNF), cAMP response element-binding (CREB) protein and Akt phosphorylation levels, and intracellular reactive oxygen species (ROS) levels were analyzed using real time PCR, Western blotting and CM-H2DCFDA assay respectively. Heat exposure under proliferation condition increased NSC/NPC viability, neurosphere diameter, and cell count. BDNF mRNA expression, CREB phosphorylation, and ROS level were also increased by heat exposure. Heat exposure increased HSP27 mRNA expression concomitant with enhanced p-Akt level. Moreover, treatment with LY294002 (a PI3K inhibitor) abolished the effects of heat exposure on NSC/NPC proliferation. Furthermore, heat exposure under differentiation conditions increased the proportion of cells positive for Tuj1 (a neuronal marker). These findings suggest that mild heat exposure increases NSC/NPC proliferation, possibly through activation of the Akt pathway, and also enhances neuronal differentiation. Direct effects of temperature on NSCs/NPCs may be one of the mechanisms involved in hypothalamic neurogenesis in heat-acclimated rats. Such heat-induced neurogenesis could also be an effective therapeutic strategy for neurodegenerative diseases.
Journal Title
PLoS One
Volume
12
Issue
12
Start Page
e0190356
ISSN
1932-6203
Published Date
2017-12-29
DOI
PubMed ID
Publisher
Public Library of Science
NII Type
Journal Article
Relation
Resource URL(HasVersion)
http://ir.lib.shimane-u.ac.jp/42528
OAI-PMH Set
Faculty of Medicine