File
language
eng
Author
Terashima, Masaharu
Takahashi, Mai
Yoshimura, Hitoshi
Mitani, Toshifumi
Tanigawa, Yoshinori
Description
Epigallocatechin-3-gallate (EGCG), a polyphenol constituent of popular beverage green tea, has been recently attracted much attention for the prevention of atherosclerosis, which is a systemic vessel disease and may lead to a variety of diseases including cardiovascular disease. Platelet-derived growth factor (PDGF) plays an important role as cell-proliferating factor in the pathogenic course of atherosclerosis, and increased activity in the PDGF signaling pathway has been implicated as a contributing factor in the progression. In the present study, to elucidate how EGCG affects the PDGF signaling pathway, we investigated the effect of EGCG on the PDGF-BBinduced activation of Ras-MAPK (mitogen-activated protein kinase) pathway, expression of immediateearly genes, and cell proliferation in rat A7r5 cells. EGCG significantly decreased PDGF-BB-induced ERK1/2 (extracellular signal-regulated kinase 1/2) and MEK1 (MAPK/ERK kinase 1) phosphorylation states dose-dependently, and also inhibited PDGFBB-induced c-fos and c-jun mRNA expressions and cell proliferation. Furthermore, PDGF-BB-induced tyrosine phosphorylation of the cognate receptor was significantly suppressed by EGCG, indicating that EGCG may act at and/or downstream the PDGF β-receptor. These results suggest that EGCG may suppress the cell proliferative signaling pathway through the inhibition of PDGF receptor kinase, MEK/ERK activity and immediate-early gene expressions. Thus, the habitual drinking of green tea may be useful to prevent atherosclerosis, and consequently decrease the mortality of cardiovascular diseases.
Journal Title
Shimane journal of medical science
Volume
22
Issue
1
Start Page
1
End Page
6
ISSN
03865959
ISSN(Online)
24332410
Published Date
2005-03-01
NCID
AA00841586
Publisher Aalternative
Shimane University Faculty of Medicine
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
Remark
http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html