| File | |
| language |
eng
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| Author |
MATSUO, Yuzy
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
KISHIMOTO, Hayafumi
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
HORIUCHI, Tomitaka
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
TANAE, Katsuhiro
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
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| Description | Gap-repair cloning for plasmid construction in budding yeast is very effective and often used. In contrast, the same method is not widely used in fission yeast, because of a shortage of information on it. Here we describe simple and effective gap-repair cloning for plasmid construction using short tracts of flanking homology. By this method, we combined concentrated DNA fragments with short (20 bp) tracts of flanking homology with the marker gene or the pre-existing gene module. In addition, we found that this method can be applied to one-step cloning of multiple DNA fragments to construct a fusion gene.
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| Subject | fission yeast
gap-repair cloning
short tracts of flanking homology
one-step cloning of multiple DNA fragments
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| Journal Title |
Bioscience, Biotechnology, and Biochemistry
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| Volume | 74
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| Issue | 3
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| Start Page | 685
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| End Page | 689
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| ISSN | 0916-8451
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| ISSN（Online） | 1347-6947
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| Published Date | 2010-03-23
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| DOI | |
| Publisher | Japan Society for Bioscience, Biotechnology, and Agrochemistry
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| NII Type |
Journal Article
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| Format |
PDF
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| Text Version |
著者版
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| OAI-PMH Set |
Faculty of Life and Environmental Science
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