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eng
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Description
The cAMP-dependent protein kinase Pka1 is known as a regulator of glycogenesis, meiosis, and stress responses in Schizosaccharomyces pombe. We demonstrated that Pka1 is responsible for calcium tolerance. Loss of functional components of the PKA pathway such as Git3, Gpa2, Cyr1, and Pka1 yields a CaCl2-sensitive phenotype, while loss of Cgs1, a regulatory subunit of PKA, results in CaCl2 tolerance. Cytoplasmic distribution of Cgs1 and Pka1 is increased by the addition of CaCl2, suggesting that CaCl2 induces dissociation of Cgs1 and Pka1. The expression of Prz1, a transcriptional regulator in calcium homeostasis, is elevated in a pka1? strain and in a wild type strain under glucose-limited conditions. Accordingly, higher expression of Prz1 in the wild type strain results in a CaCl2-sensitive phenotype. These findings suggest that Pka1 is essential for tolerance to exogenous CaCl2, probably because the expression level of Prz1 needs to be properly regulated by Pka1.
Subject
Pka1
Prz1
calcium tolerance
Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume
81
Issue
2
Start Page
231
End Page
241
ISSN
1347-6947
ISSN(Online)
0916-8451
Published Date
2016-10-19
DOI
Publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry
NII Type
Journal Article
Format
PDF
Text Version
著者版
Gyoseki ID
e31382
OAI-PMH Set
Faculty of Life and Environmental Science
Remark
This is an original manuscript / preprint of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2016-10-19(online), available online: http://www.tandfonline.com/10.1080/09168451.2016.1246171