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Title Transcription
ダイチョウキン ユライ Lガタ キン NC7 ニオケル サイボウ ヨウセキ ノ チョウセツ
Title Alternative
Regulation of Cell Volume in Escherichia coli : L-form NC7
File
language
eng
Author
Onoda, Tetsuo
Tanaka, Hiroyuki
Description
The ATPase inhibitor dicyclohexylcarbodiimide (DCCD), which collapse the chemical and electrical components of the proton motive force, caused rapid cell swelling. Addition of extracellular osmotic salt such as CaCl_2 prevented the swelling of DCCD-treated cells incubated in NaCl solution. Ionophore nigericin, which collapse the proton gradient by promoting electroneutral K+ / H+ exchange , and protonophore carbonylcyanide-m-chlorophenylhydrazone(CCCP, 10 μM) induced osmotic swelling in NaCl solution, but not in CaCl_2.External solutes such as CaCl_2 prevented the swelling of DCCD-treated cells incubated in NaCl. Ionophore valinomycin , collapse membrane potential in the presence of KCI, falled to induce swelling in NaCl solution. These results show that in L-form NC7 the swelling was induced by the collapse of the electrochemical gradient of proton. Therefore, it was postulated that Na+ that diffused into the cell was extruded by a Na+ / H+ antiport energized by the proton motive force to maintain constant cell volume.
Journal Title
Memoirs of the Faculty of Science, Shimane University
Volume
25
Start Page
63
End Page
72
ISSN
03879925
Published Date
1991-12-25
NCID
AN00108106
Publisher
島根大学理学部
Publisher Aalternative
The Faculty of Science, Shimane University
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Shimane University
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