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Title Transcription
コウ アルカリセイ BACILLUS ASSC-2 ノ チュウセイ PH ヒ カンジュセイ トツゼン イヘン カブ ニツイテ
Title Alternative
A Neutral pH-insensitive Mutant of Alkalophilic Bacillus sp. ASSC-2
File
language
eng
Author
Onoda, Tetsuo
Description
Intracellular pH of an alkalophilic Bacillus sp. ASSC-2 was acidified in the K^+-limited media and could not grow at neutral pH range unless K^+ was added to the media (Oshima & Onoda, 1990). The neutral pH-insensitive mutant, Bacillus ASSC-2NI, was isolated by the treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The mutant could grow in the K^+-limited media even at neutral pH range in the absence of exogenously added K^+, and the intracellular pH was not acidified. The mutant has gained high K^+-transport activity and,consequently, the respiratory activities of the mutant were higher than the parent strain in the absence of added K^+ at neutral pH range. Further, the mutant has retained Na+/H+-antiporter activity at pH 7. The properties of the mutant indicated that if K^+-transport system was actively operating at neutral pH the intracellular pH was protected from acidification by the active Na + /H+-antiport system at neutral pH.
Journal Title
Memoirs of the Faculty of Science, Shimane University
Volume
25
Start Page
73
End Page
83
ISSN
03879925
Published Date
1991-12-25
NCID
AN00108106
Publisher
島根大学理学部
Publisher Aalternative
The Faculty of Science, Shimane University
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Science and Engineering
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