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Title Transcription
ダイチョウキン L−FORM NC7 ノ ダクド ヘンカ ト ゾウショク ニ オヨボス シントウテキ アンテイザイ ノ コウカ
Title Alternative
Effects of osmotic stabilizers on turbidity changes and growth of Escherichia coli L-form NC7
File
language
eng
Author
Onoda, Tetsuo
Description
The effects of osmotic stabilizers on swelling and growth of L-form NC7 derived from Escherichia coli K 12 were examined. The presence of impermeable solute , such as NaCl or KCl, in the suspended medium were effective as osmotic stabilizer, while permeable solute, such as glycerol were ineffective . On the other hand, when sucrose was used as osmotic stabilizer, in cells upshocked with 0.2 M sucrose, rapid decrease in optical density and then slow restoration were observed. In the upshocked cells, potassium ion had protective effect against changes of turbidity. From colony forming units at 1 hr after upshock, it was shown that there was no srgnificant different between cells upshocked with 0.2 M sucrose alone and with 0.2 M sucrose plus 0.34 M KCl. Low osmolarity (0.17 M) of cation, such as NaCl or KCl, was not sufficient to protect fragile L-form cells unless 0.2M sucrose was added in the incubation medium. In combination with NaCl and KCl at high concentration (0.4M), there are competition between Na and K ions on growth, suggesting that in the combination with calcium (1 mM), Na ion also play a important role on growth of L-form NC7 as well as K ion.
Journal Title
Memoirs of the Faculty of Science, Shimane University
Volume
22
Start Page
93
End Page
101
ISSN
03879925
Published Date
1988-12-25
NCID
AN00108106
Publisher
島根大学理学部
Publisher Aalternative
The Faculty of Science, Shimane University
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Shimane University
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