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language
eng
Author
GUPTA, Dipali Rani Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
PAUL, Swapan Kumar Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
OOWATARI, Yasuo Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
Description
Nine sam mutants that undergo sexual differentiation without requiring starvation in Schizosaccharomyces pombe were previously isolated. In this study, we identified a nonsense mutation on the pka1 locus in the sam6 mutant. pka1 encodes a catalytic subunit of protein kinase A (PKA). Replacement and overexpression of pka1 suppressed the KCl sensitivity and hyper-mating phenotype of sam6, confirming that sam6 is an allele of pka1. To characterize further the regulation of Pka1, we tested the physical interaction between Pka1 and Cgs1 (a regulatory subunit of PKA). Pka1 and Cgs1 physically interacted under glucose-limited conditions but not under glucose-rich conditions. In addition, the formation of a Pka1-Cgs1 complex was detected under glucose-limited conditions by Blue Native PAGE. Furthermore, the Pka1 protein was found to be phosphorylated under glucose-starved conditions, and at the same time its localization shifted from the nucleus towards the cytoplasm (mainly the vacuoles), suggesting a strong relationship among phosphorylation, complex formation, and the cytoplasmic distribution of Pka1.
Subject
fission yeast
Schizosaccharomyces pombe
protein kinase A
sexual differentiation
Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume
75
Issue
8
Start Page
1456
End Page
1465
ISSN
0916-8451
ISSN(Online)
1347-6947
Published Date
2011-08-23
DOI
Publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry
NII Type
Journal Article
Format
PDF
Text Version
著者版
OAI-PMH Set
Faculty of Life and Environmental Science
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