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language
eng
Author
Arai, Mary
Ihara, Setsunosuke
Description
Reepithelialization of skin which comprises epidermis and dermis has not been fully elucidated due to the complexity of the participants as well as the interactions therein. In this study, the intrinsic roles and behaviors of epidermis itself dur- ing wound closure on neonatal rat skin were explored by developing and utilizing a novel in vivo wound model, termed “shallow incisional wound” in which the injury of dermis was minimized. The shallow wounds were closed by 12 h postwounding (PW) by the migration of the wound-marginal epidermal sheets in which activated myosin light chain (p-MLC) was predominantly detected at the lateral plasma membrane of individual cells. By local administration of Rho-associated protein kinase (ROCK) inhibitor Y27632, p-MLC disappeared at the wound margin and wounds were not closed by 12 h PW. Inhibition of Rac 1 by NSC23766 also resulted in hold of wound closure by 12 h PW, though NSC23766 somewhat slowly acted on p-MLC expression. These results suggest that, without joining of dermis, epi- dermal cells have a potential ability of closing wounds by active epithelial sheet movement integrated by Rho family small GTPases-dependent extension and contraction of the individual cell bodies.
Subject
Wound Closure
Reepithelialization
Rat
Neonate
Epidermis
Migration
Actomyosin
Rho Family Small GTPases
Journal Title
CellBio
Volume
2
Issue
4
Start Page
257
End Page
266
ISSN
23257776
Published Date
2013
DOI
Publisher
Scientific Research Publishing
NII Type
Journal Article
Format
PDF
Text Version
出版社版
Gyoseki ID
e20401
OAI-PMH Set
Faculty of Life and Environmental Science
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