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language
eng
Author
Yamamoto, Takaharu National Institue of Information and Communications technology, BIO ICT Kobe, JP 651-2492
Kobayashi, Yasuyo Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue 690-8504, Japan
Guo-Lei Zhou Department of Biological Sciences, Arkansas State University, State University, AR 72467, USA
Description
Schizosaccharomyces pombe Cap1 has been identified as the (adenylyl) cyclase associated protein. Cap1 was able to form homomers , and also binds actin. Cap1 localized to the growing tip, and this localization was dependent on the the P2 region in the middle domain. In a two-hybrid screening using cap1 as a bait, we isolated csh3, which encodes a protein of 296 amino acids with an SH3 domain and a proline/glutamine rich region. The binding of Csh3 and Cap1 was confirmed by in vivo pull down assays. Cooperative functions of Csh3 and Cap1 were observed. Deletion of both csh3 and cap1 resulted in heightened sensitivity to CaCl2, while disruption of either gene alone did not have any effect in this regard. In addition, over-expression of csh3 or cap1 alone did not affect cell growth, while over-expression of both genes resulted in growth retardation. Finally, while Csh3-GFP localized to the cytoplasm in wild-type cells, its localization was altered in cap1Δ cells, suggesting that the interaction between Csh3 and Cap1 controls the cellular localization of Csh3. These results demonstrate that Cap1 in S. pombe is a multifunctional protein that functions through dimerization (or multimerization) and interaction with other proteins including adenylyl cyclase, actin and a protein Csh3.
Subject
S. pombe
CAP
Csh3
Journal Title
FEMS yeast research
Volume
15
Issue
8
ISSN
1567-1364
Published Date
2015-11-04
DOI
Publisher
Amsterdam ; New York : Published by Elsevier Science B.V. on behalf of Federation of European Microbiological Societies, [2001-
NII Type
Journal Article
Format
PDF
Rights
https://academic.oup.com/femsyr
Text Version
著者版
OAI-PMH Set
Faculty of Life and Environmental Science
Remark
FEMS Yeast Research, Volume 15, Issue 8, December 2015, fov097, https://doi.org/10.1093/femsyr/fov097
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