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Title Transcription
シジョウセイ コウ オンセイ ランソウ PHORMIDIUM LAPIDEUM ノ セイゲン コウソ
Title Alternative (English)
Restriction Endonucleases from Phormidium lapideum, a Strain of Filamentous and Thermophilic Cyanobacteria
File
language
eng
Author
Ochiai, Hideo
Shibata, Hitoshi
Sawa, Yoshihiro
Ashida, Naomi
Description
A couple of restriction endonucleases, Pla I and Pla II, have been purified from a filametous and thermophilic cyanobacterium, Phormidium lapideum. Pla I was proved to be an isoschizomer of Hae III to cleavage the site GG↓CC and was a monomeric protein that had a molecular weight of about 40 kilodaltons. Pla II was an isoschizomer of Nsp (7524) V to recognize the site TTCGAA and was estimated as a heterotetrameric protein (α_2β_2). Pla II has an apparent molecular mass of 176 kilodaltons and that of α subunit was 63 kilodaltons, β subunit 31 kilodaltons. Characteristics of Pla I and Pla II were investigated in comparison with that of the respective isoschizomers, Hae III and Nsp (7524) V.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
23
Start Page
184
End Page
191
ISSN
0370940X
Published Date
1989-12-21
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Life and Environmental Science
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