Title Transcription | シジョウセイ コウ オンセイ ランソウ PHORMIDIUM LAPIDEUM ノ セイゲン コウソ
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Title Alternative (English) | Restriction Endonucleases from Phormidium lapideum, a Strain of Filamentous and Thermophilic Cyanobacteria
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File | |
language |
eng
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Author |
Ochiai, Hideo
Shibata, Hitoshi
Sawa, Yoshihiro
Ashida, Naomi
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Description | A couple of restriction endonucleases, Pla I and Pla II, have been purified from a filametous and thermophilic cyanobacterium, Phormidium lapideum. Pla I was proved to be an isoschizomer of Hae III to cleavage the site GG↓CC and was a monomeric protein that had a molecular weight of about 40 kilodaltons. Pla II was an isoschizomer of Nsp (7524) V to recognize the site TTCGAA and was estimated as a heterotetrameric protein (α_2β_2). Pla II has an apparent molecular mass of 176 kilodaltons and that of α subunit was 63 kilodaltons, β subunit 31 kilodaltons. Characteristics of Pla I and Pla II were investigated in comparison with that of the respective isoschizomers, Hae III and Nsp (7524) V.
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Journal Title |
Bulletin of the Faculty of Agriculture, Shimane University
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Volume | 23
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Start Page | 184
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End Page | 191
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ISSN | 0370940X
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Published Date | 1989-12-21
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NCID | AN00108015
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Publisher | 島根大学農学部
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Publisher Aalternative | Shimane University, Faculty of Agriculture
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NII Type |
Departmental Bulletin Paper
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OAI-PMH Set |
Faculty of Life and Environmental Science
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他の一覧 |